Natural Science - Year II
Unit 60 Lab: Matching DNA strands
Lab for Unit 60
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Unit 60 Laboratory Activity: DNA Analysis
Goal: Compare modern human DNA with the DNA of primates
Materials
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Website: DNA BLAST Sequence Analyzer Websites
Procedure
- Select the Nucleotide Structure Sequences database under "Structure".
- Cut and paste each of the following two sequences (one at a time) into the DNA text area on the tool you choose. Be sure to remove new lines or spaces if you have any
- GTTNNTTATNAAGTAGNGCCCTTATATAAACTATTCTCTGTTCTTTCATG
GGGAAGCAGATTTGGGTACACCCAAAGGTATTGACTCACCCATCAACAAC
CGCTATGTATTTCGTACATTACTGCCAGCCACCATGAATATTGTACGGTA
CCATAAATACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAA
ACCCCCTCCCCATGCTTACAAGCAAGTACAGCAATCAACCCCCAACTATC
ACACATCAACTGCAACTCCAAAGCCACCCCTCACCCACTANGATACCAAA
NAAACCTACCCACCCTTAACAGTACATAGCACATAAAGCCATTTACCGTA
CATAGCACATTACAGTCAAATCCCTTCTCGTCCCATGGATGACCCCCCTC
AGATAGGGGTCCCTTGACCACCATCCCCA
- NGGNCNNCTTTTNNNATTCTTNCCGATTGGTCCTTTAAAACNCTTGCNCN TNGGGCCCGGGTAANNCTACAACTTCCTNCNTTGGCTACNGTTNNCCNNC NGCTTTCTNTNTCNNACNANACAGTTCGCCTNTTTTCANTNTTTNACGTG CNCNNNAANACNANNCCNNATNTGGTGGGTCCATTCTNGCNACTCTCNNA AATCCCNGTCGNCCTNGGTCTTTNTGNNTCCTCNNTNTTNNCTNCNNNTT CCCCNNGAANCTNNNNNGCNNNNNTCCNCCCTCTNNCNCTNANCTANNGN CCCNCTNTNTCNGCCTTNTANTCCTNCGTATTNTGGCNTTGGCAGNACAN TNCNCTTTANTTNCGNCTCNTNCCNCATCCCCATGNAGNTACCCCCCTCG GTNGGNGGGCTCAGCATGNCNANAAATTGTCATTTNTAAGGTNTTTCCNT CTCCNANTTCCTCGNTATAATTTNNTGTGACCTGGGAGTGTTTTTNTNNT TNCNGGTNTGNNCCNTCTGNNNGNAAATTTTCCCNGCCCCGNNTGNTNTN TTTTTTTGTTCCNCNGNTATACCATCNCCATCCCTTGCTNNGTNTTTNNT TTNTNTTTATATCNTCCTCTTCGTNTTTNTTCCCCTTTGCTCCCNNGGGG GGCCANANNTNTNNCNNNNGATAATTCCTCNGGNTGGNCNCATACNTTTG GTTCCNGCNTTTNTTTGCTCCTCTTCCTANGGGGTTGGGNCCTCNTNTTA TTTCNNTTNCCCTTNNCTNGGTGTTTTCNTTNNCCTCNNGGGGNNCTTTT TTTTCCTCCCNNNCNCTTCANNACCCTTCCTTTCTNCCCNCTCCTTTCCT NTTTTTTTGNNNNTTCTTNCTCCTNCCCCNCTNGTCTTCGTTCTTTTNTT TCTTCCNTCTCCTCTTCTTCN
- GTTNNTTATNAAGTAGNGCCCTTATATAAACTATTCTCTGTTCTTTCATG
GGGAAGCAGATTTGGGTACACCCAAAGGTATTGACTCACCCATCAACAAC
CGCTATGTATTTCGTACATTACTGCCAGCCACCATGAATATTGTACGGTA
CCATAAATACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAA
ACCCCCTCCCCATGCTTACAAGCAAGTACAGCAATCAACCCCCAACTATC
ACACATCAACTGCAACTCCAAAGCCACCCCTCACCCACTANGATACCAAA
NAAACCTACCCACCCTTAACAGTACATAGCACATAAAGCCATTTACCGTA
CATAGCACATTACAGTCAAATCCCTTCTCGTCCCATGGATGACCCCCCTC
AGATAGGGGTCCCTTGACCACCATCCCCA
- Leave the program defaults at blastn and start the comparison. It may take several minutes to run.
- Record the number of matches at 95% or better with human DNA.
Report
Describe your results: how many sequences did the sample DNA strand match against known human DNA?
Challenge question: How might the results of your investigations be used to support theories of evolution?
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