Lab for Unit 58
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Do not handle the coffee filters any more than necessary, and be sure that your hands are clean and dry before working with it. Skin oils will interfere with the separation.
The banding is characteristic of the pigments, and can be used to identify them in different plants. In spinach, the pigments are carotene (yellow/yellow orange), xanthophyll (yellow), chlorophyll a (bright green to blue green), and chlorophyll b (yellow green to olive green). You can use the spinach bands to identify the banding in other plants.
Describe your procedure, the solvents you use, and the source of your pigments. Describe the bands which result as the solvent separates the pigments. How many pigments show up in your sample? Which solvent works best?
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